BIOS 210 Lecture 2 - Nucleic Acids o They are polymers...

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Nucleic Acids o They are polymers Monomers are nucleotides Nucleotides have three parts Nitrogenous base (A, T, G, C, U) Sugar molecule (ribose or deoxyribose) Phosphate group o Two main types DNA (deoxyribonucleic acid) RNA (ribonucleic acid) o They are relatively stable Good for carrying genetic information! o Experiment 3-1: Extracting and Digesting DNA Extracting nuclei from wheat germ cells Eukaryotes- DNA is in nucleus inside cell Separate nucleus from rest of cell through centrifugation o Centrifuge spins sample at high speed o Makes bigger particles (nuclei) settle to the bottom o Material at the bottom- pellet o Liquid left on top- supernatant Stabilize nucleus with extraction buffer Extracting DNA from nuclei DNA is still in nucleus- confirm with methylene blue o Methylene blue dyes DNA o Nuclei should appear as blue blobs Add EDTA and SDS to test tube (weakens nuclear membranes) DNA should be out of the nucleus- confirm with
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BIOS 210 Lecture 2 - Nucleic Acids o They are polymers...

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