2013 FA BIMM100 Midterm 1 practice #2_key - Question 1 Replication Name RNA primer 3 5 GUCCAGCGUU CGATAATCAAT 3- 5 DNA Above is shown a schematic of a

2013 FA BIMM100 Midterm 1 practice #2_key - Question 1...

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Question 1. ReplicationName:____________________ Page 2 Above is shown a schematic of a DNA strand annealed to an RNA primer produced by Primase during replication. 3’ --AGGCTACGCAGGTCGCAAGCTATTAGTTA-- 5’ GUCCAGCGUU1b)On the schematic on the top of the page, label which strand is DNAand which is the RNA primer. (2). 1c)5’ and 3’ ends are shown for one of the strands on the schematic on the top of the page. Label 5’ and 3’ ends of the other. (2). 1e)If the schematic shown above represents a lagging strandduring replication, draw an arrowbelow to show the direction of movement of the replication forkthat is engaged in replicating the shown DNA. (2)1f)When the nextsegment of the lagging strand is replicated, DNA polymerase stops when it runs into the RNA primer. In the order in which they are used, list the three types of enzymes that are used to replace the RNA primer with DNA. (3)Direction of movement of replication fork (show arrow here):Three types of enzymes used (in order used):1: _______2: _______3: _______ RNA primer DNA 3’ 5’ RNase (H) DNA polymerase 1d)On the schematic at the top of the page, fill in the sequence added by DNA polymerase as it replicates from the shown RNA primer. (3). 1a) During which phase of the eukaryotic cell cycle does Primase produce RNA primers? (2).
Question 2. DNA repairName:____________________When DNA polymerase makes mistakes during replication and inserts the wrong nucleotide, it can repair the mistake itself using its exonuclease proofreading activity. Page 3

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