BIMM100 Problem Set 1 answers_JL_TAs(1)

Dna polymerase nuclease dna ligase 3a write

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Unformatted text preview: ed that the percentages of the bases A, C, T, G, Y, and Z, respectively, are 10%, 17%, 10%, 17%, 23%, 23% Does the distribution of the nucleotides follow Chargaff’s rules? If so, what does this imply about Y and Z??? Yes. This would imply that Y and Z are base pairing. 2c. If this organism uses three bases in a single codon, what is the maximum number of possible codons? 6*6*6= 216 maximum unique codons 2d. During initiation of replication, a DNA helicase begins to unwind the double stranded DNA. What is the name of this starting point? Origin a. After a moment of unwinding, primase synthesizes RNA primers. Draw a partially unwound double stranded DNA and mark the location of the primer with red arrows; make sure to indicate 5’ and 3’ ends of the DNA and primers. b. The DNA helicase further unwinds the template DNA and DNA polymerase synthesizes complementary strands. Draw the unwound template DNA with red arrows indicating primers and green lines indicating newly synthesized strands; make sure to indicate 5’ and 3’ ends. c. Besides containing leading strands, replicating DNA also contains ____________ strands. Draw the primer for this strand, in addition to the structures listed in part (b). lagging d. In order to complete the complementary strand, _____________________ is used to synthesize the complementary lagging strand, _________________ is used to excise RNA primers, and after DNA is synthesized in place of the primers, ___________________ is used to “glue togeth...
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