BIMM100 Problem Set 1 answers_JL_TAs(1)

What does this mean for the mechanism by which the

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Unformatted text preview: y labeled phosphate, which correlates to the presence of DNA. c. Because this is a completely foreign pathogen, scientists decide to study the manner in which the pathogen replicates. Scientists generate the virus in the presence of 15N and, after infection of cells in the presence of 14N, allow the pathogen to replicate twice. The scientists then ultra- centrifuge the pathogen DNA through a CsCl gradient and discover that all DNA has a density corresponding to ¼ 15N and ¾ 14N. What does this mean for the mechanism by which the virus replicates? This means that replication of the virus is Dispersive. d. Hypothetically if scientist observed that there is only light weight and heavy weight 15N labeled DNA, but no mixed weight DNA, what can he or she conclude? That replication is conservative. 1c. You characterize and culture two distinct strains of a xenobiotic microbe from Mars, one of which is yellow and the other green. You find that incubating the live yellow microbe with a heat inactivated green microbe causes a subset of the yellow microbes to turn green. However, the reverse experiment does not produce a change in the green microbe. You conclude that genetic material is being transferred from the dead green microbe to the live yellow microbe. Since these are alien life forms you are unsure of what the genetic material is. You set out to test for what the genetic material is in this life form by treating the remains of the dead green microbe with various enzymes to degrade protein...
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