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4-1-13_frogs_pt_3 - The Organizer inhibits BMPs and Wnts to...

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adapted from Gilbert 9e, Fig. 7.27 Noggin and Chordin bind BMPs and render them ineffective The Organizer inhibits BMPs and Wnts to achieve neural induction in the ectoderm FrzB, Dickkopf, and Cerberus bind Wnts and render them ineffective Wnt8
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BMPs and Wnts normally make ectoderm epidermal Control - Sox2 BMP2,4,7 + ADMP MO - Sox2 Conclusions : (1) Without ventral signals, ectoderm becomes neural. (2) BMPs lead to epidermis. (3) Neural ectoderm results from neutralizing ventral signals. The Organizer accomplishes this. Gilbert 9e, Fig. 7.28 Only the neural plate expresses Sox2 normally All ectoderm expresses Sox2 in the absence of BMP signaling
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adapted from Gilbert, 9e, Fig. 7.6 Gastrulation changes the spatial relationship of Organizer to ectoderm Initially, the Organzer mesoderm lies at the surface. At this time, it could be sending signals along the surface to the ectoderm (planar) .
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Gastrulation changes the spatial relationship of Organizer to ectoderm Later, the Organzer mesoderm lies under the ectoderm . At this point it could be sending signals outward ( vertically ) to the ectoderm. In the posterior , planar signals could still operate adapted from Gilbert, 9e, Fig. 7.6
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adapted from Wolpert 2e, Fig. 4.21 Keller explants show that “planar” induction can occur Pieces of the early gastrula that include what would normally become neural ectoderm and axial mesoderm ( notochord and somites ) are excised. A practical issue: these are not happy on their own, so two of these are placed together back-to-back.
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adapted from Wolpert 2e, Fig. 4.21 Keller explants show that “planar” induction can occur mesoderm neural ectoderm • If neural ectoderm forms, the only type of signals it can receive are planar signals, since the mesoderm lies alongside ectoderm. • Neural markers are expressed. Conclusion : planar signals can induce neural ectoderm. ? only planar signals can occur
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Wolpert 2e, Fig. 4.20 Mangold’s “Einsteck” (insertion) experiment shows that “vertical” induction can occur A A P P Anterior mesoderm placed into early gastrula induces head with eyes and forebrain Posterior mesoderm placed into early gastrula induces a trunk and tail • The only type of signals involved are vertical signals. • Neural structures form appropriate to the type of mesoderm implanted. Conclusion : vertical signals can pattern neural ectoderm.
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Neural Induction: Summary Gilbert 9e, Fig. 7.35 In the trunk, BMP inhibitors result in future spinal cord . Wnt inhibitors lead to future brain (anterior neural tube). Wnts, FGF & retinoic acid (RA) posteriorize the spinal cord.
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cerberus promotes brain derivatives Gilbert 8e, Fig. 10.37 mRNA overexpression of cerberus leads to formation of ectopic brain structures
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Neural induction involves multiple signals Gilbert 9e, Fig. 7.30
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Spemann: The Organizer Gilbert 7e, Fig. 10.21 The organizer forms on the dorsal side of the embryo.
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