443 Lecture 17 building a new ship II (1)

Mostcommonc14

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Unformatted text preview: ng in inner piece of membrane LPS'(Endotoxin)' •  Three'regions' –  Lipid'A:'diMglucosamine'with'all'hydroxyls'and'amines'subsOtuted'with' faIy'acids.'Most'common'C14.'' –  Core'with'two'regions;''Inner,'most'conserved'KDO;'outer,'more'variable' –  O'anOgen:'Repeat'carbohydrate'units;'confers'immunological'specificity' CompleOon'of'Core' membrane cytoplasm pattern will vary there will be a set of sugars that forms the outer core it is built off of KDO KDO + sugars represents the core biosynthetic reaction occurs in the cytoplasm OManOgen'synthesis' can move accross the membrane then attach the o antigen start with lipid A attach core (which KDO is first sugar + other sugars) then add O antigen repeating unit gets attached to undecaprenyl carrier and then gets transfered across the membrane then its added to LPS Transfer' Synthesis'of'' across' repeaOng'unit' (on'Undecaprenyl'Carrier)'' membrane' AddiOon' to'LPS' LPS'MaturaOon' looks kind of like GPS system of Type 2 system got a carrier protein that moves it across ..1. anytime you are assembling sugars into polymer its occuring in the cytoplasm attaching to something in the membrane and moving it (lipid A +core) then attach O antigen attach O antigen when its on the periplasmic side of the cytoplasmic membrane protein mediated process that moves it to the outer membrane proteins work well for Salmonella or E-coli but for other bacteria its different names no one knows yet if the left or right side is the active model to move from bottom to top requires a protein process. FOR LPS SYNTHESIS: make lipid A attach KDO move across membrane attach O antigen protein mediate process to move across the membrane. LPS'(Endotoxin)' •  Three'regions' –  Lipid'A:'diMglucosamine'with'all'hydroxyls'and'amines'subsOtuted'with' faIy'acids.'Most'common'C14.'' –  Core'with'two'regions;''Inner,'most'conserved'KDO;'outer,'more'variable' –  O'anOgen:'Repeat'carbohydrate'units;'confers'immunological'specificity' Overview'of'Biosynthesis' Macromolecule'1' Precursor(s)'''' Subcomponent'1' Precursor(s)' Subcomponent'2' Precursor(s)' Subcomponent'3' Building'Block(s)' Macromolecule'2' Capsules/Slime'layers' •  aka'glycocalyx' •  C...
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