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Unformatted text preview: ic reaction. must have an aploretic reaction to get glyoxylate shunt. 50% of energy in cell going into protein synthesis. part of that is the
-if bacterium makes propionic acid, it probably won't make butynol.
glutamate. 1. it is a requirement. 2. have
-acetolactate pathway-example Bacillus to have enzyme or else cant carry out reaction. not every bacteria will be able to do all three pathways. most can do
atleast one. its part of the anaploretic reaction. storage polysaccharide. steps. Other example make acetyl coA or way for bacterium to get through the hard
-what about phbaformation should we know? its adon't need to know exactmake when theres a surplus of carbon-its amediium-devoid of phosphate-TCA cycle.
Only it is to off shoot of fatty acid synthesis. has condensation step. don't care about speciﬁc steps. get refractile cells. ﬂexible. can do lots of different things.
times. way an do so is the methyl-glyoxylate pathway-make pyruvate without consuming phosphate. Bacteria are hugely some bacteria are very efﬁcient in making
penvironment is generally nutrient poor-thats how you survive in environment in nutrient poor. (that has no phosphate)
hb. if they have genes then they will store it.
-continuous culture- reduce contamination-sufﬁcient inoculum to allow that bacterium to dominate your culture. if you have an inoculum that is contaminated,
that other bacterium can take over your culture if it has a better doubling time than the one of interest.
-beta oxidation-a way in which you can take fatty acid that is ubiquitous in the environment.by deﬁnition a cell will have phospholipid bilayer that makes cell
membrane (will attach to fatty acid). core of glycerol + fatty acids. (ex. butter) how to use those to extract energy. beta-oxidation involves multiple steps. pull 2
carbon unit off at a time. reverse of synthesis. if you start with c16 fatty acid -> going to create c14 fatty acid + acetyl coA + electrons (FADH2 + electrons)
acetyl coA will feed into TCA cycle (aerobes)
-specialized group of organisms that can carry out beta oxidation. not all bacteria can do it. practical energy (bioremediation-clean up o...
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