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Unformatted text preview: 'arOcle?'
General'layout'of'the'presentaOon' ﬁnal presentation due Wednesday before
Thanksgiving Format: short answer questions
-identity of fermentation products (3 routes, anaploretic, acetyl CoA pathway, acetyl-lactone pathway) know each. different bacteria do different things.
-vary rarely do you get one fermentation product out of it. you usually get a mixture of products. most common=heterofermentation. don't always make it at time 0.
have to have a modﬁﬁcation in the culture. sometimes culture H sometimes culture ph. all inﬂuence which products are made for the bacterium.
-claustridia will make byutnol. never make 100% butynol
-culture-3 types (take colony from a plate, add at time 0, see a lag, exponential growth, stationary phase, then cells die off-BATCH), a continuous culture is used in
almost every industrial process, you're not limited by the lag phase or the exponential phase. you make start with ﬁxed innoculum. then feed media, draw out a
volume of media, do at same rate, media in =media out, advantage-bypass lag and exponential phase, gives you what you're interested in. batch culture-1 million
Liters (reduce level of contamination)-need large innoculum. 10^9 cells start with. On the other hand with continuous cultures, 100,000 Liter, 10000, 1000, 10 L…
takes longer but process is continuous. as long as you are sterile going in and going out, cultures can last for months. just replace the medium. feed media, pull out
what you want. Last one is synchronous-add inoculum. NOT on exam. synchronous good to study the cell cycle. period of time [not 100%] synchronous culture-use
small amount, follow something like batch, follow some form of uniform division process. In a synchronous culture, a batch is probably more of a model than
-for fermentation 3 major pathways. know a bacterium that does each.
-glyoxylate shunt-anaploretic reaction (L9) every bacterium in a genus carries out entire process. they may carry make malate,
-not every bacterium does every process. notstart with c6 intermediate, split it, enzyme unique to glyoxylate shunt, out part of it. make succinate. It is an option
for an anaploret...
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