443 Lecture 15 Growth (1)

energy needed for homeostasis and other essenfal

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Unformatted text preview: omposiFon –  C, N, P etc –  Ease of uFlizaFon •  Rich medium •  Minimal medium Precursor shunts C6 Sugar Carbohydrates Energy(2) P ­C6 Sugar ­P C3 Sugar PPP CO2 Energy Precursors G6P F6P Precursors R5P E4P Precursors T3P PGA PEP PYR Precursor AcCoA Precursors KG Suc OAA C3 Sugar Energy (varies) C3 Energy (6) CO2 C2 Energy (24) CO2 Growth k(food ­>CO2) > l(precursors)  ­ m(macromolecule syn)  ­ n(ess. meta.) k(food ­>CO2) – Energy available if food completely converted to energy l(precursors)  ­ Energy lost due to the shunt of a metabolite to macromolecule synthesis or storage m(macromolecule syn) – Energy needed to convert precursors into building blocks and the energy needed to convert the building blocks into macromolecules. Key variable: maintenance physiology + synthesis of new macromolecules n(ess. meta.)  ­ Energy needed for homeostasis and other essenFal acFviFes of the cell Effect of NutriFon on Growth Rate Salmonella typhimurium Medium Feature Growth Rate (g in min) Nutrient broth Very rich 22 All aa Complete mix + salts 32 ParFal aa set 8 aa + salts 49 Glucose minimal Glucose, NH3 + salts 50 Succinate minimal Succinate, NH3 + salts 64 Asp minimal Asp + salts 72 Lys minimal Lys + salts 97 Things that impact growth rate •  Bacterium •  Medium composiFon •  Growth condiFons –  Temperature –  pH –  Salt –  Gas availability Temperature & pH ranges of bacteria Type of Organism Growth Temperature (°C) Min Opt Max Psychrophilic  ­5  ­ 0 5  ­ 15 15  ­ 20 Mesophilic 10  ­ 20 20  ­ 40 40  ­ 45 T...
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