443 Lecture 15 Growth (1)

443 Lecture 15 Growth (1) - BSCI 443 MICROBIAL PHYSIOLOGY...

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Lecture’15’ Growth’ BSCI’443’MICROBIAL’ PHYSIOLOGY’
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Last’Time’ Autotrophy’ Carbon’fxaFon’ RuBISCO’ Reverse’EMP’ Reverse’PPP’ Energy’requirement’ Energy’Input’ Membranes’ Chemolithotrophy’ Reverse’Electron’transport’ PhotosyntheFc’ Photosystems’ Three’types’ Energy’conversion’
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Growing’and’Dividing’Cells’ Quest for food Sensing’the’ environment’ Movement’ U,liza,on of food AcquisiFon’ Metabolism’ Energy’ Building’blocks’ Growth Synthesis’of’ macromolecules’ Cell’structures’ Cell’division’ Stress’ The community Cross’talk’ Bio±lms’ ²ight’for’food’
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Batch’Culture’Growth’ Time’ Ln’(number’of’cells)’ lag’ log’ (exponenFal)’ staFonary’ death’
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hSp://www.scripps.edu/mb/goodsell/illustraFon/public/’ Growth ’is’an’orderly’increase’in’the’quanFty’ of’cellular’consFtuents.’ Number’of’cells’ Volume’ Increase’cell’ consFtuents’ Growth’
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Cell’Division’ Binary’fssion’ Cell’elongates’(increase’in’volume)’ Chromosome’duplicates’ Septum’Forms’ Cell’divides’
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Fig. 6-3 Cell wall Cytoplasmic membrane Nucleoid MinE Divisome complex FtsZ ring Nucleoid Septum MinE Minutes 0 20 40 60 80
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Other’types’of’Cell’Division’ Filamentous’Gram’+’ Hyphomonas Caulobacter Pasteuria Cyanobacteria’ Streptomycetes
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Batch’Culture’Growth’ Time’ #’cells,’cell’volume,’Total’protein’ lag’ log’ (exponenFal)’ staFonary’ death’
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Measurement’of’Growth’ 1.’Direct’ physical measurement ’of’dry’weight,’wet’ weight,’volume’of’cells’a\er’centrifugaFon,’or’ counts’of’the’number’of’cells.’ Method Applica,on Comments Direct’microscopic’count’ EnumeraFon’of’bacteria’ May’not’disFnguish’living’from’ nonliving’cells’( tryptan’blue)’ Viable’cell’count’(colony’ counts)’ EnumeraFon’of’bacteria’in’ milk,’foods,’soil,’water,’ laboratory’cultures,’etc.’ Very’sensiFve’if’plaFng’ condiFons’are’opFmal’ Measurement’of’dry’weight’or’ wet’weight’of’cells’or’volume’ of’cells’a\er’centrifugaFon’ Measurement’of’total’cell’yield’ in’cultures’ probably’more’sensiFve’than’ total’N’or’total’protein’ measurements’
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Measurement’of’Growth’ 1.’Direct’ physical measurement ’of’dry’weight,’wet’ weight,’volume’of’cells’a\er’centrifugaFon,’or’ counts’of’the’number’of’cells.’ 2.’Direct’ chemical measurement ’of’some’chemical’ component’of’the’cells,’such’as’total’N,’total’protein,’ or’total’DNA’content.’ .’ Method Applica,on Comments
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443 Lecture 15 Growth (1) - BSCI 443 MICROBIAL PHYSIOLOGY...

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