443 Lecture 15 Growth (1)

693 t t0ln nt ln n0 g generafon fme h t0 fme zero

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Unformatted text preview: replicaFon Cell Cycle A 2A Chromosome replicaFon Cell division 1 2 Cell Cycle Chromosome replicaFon Chromosome replicaFon Chromosome replicaFon Cell division Cell division Cell division Log (number of cells) Growth Rate Time Examples of GeneraFon Times Bacterium Medium Genera,on Time (minutes) Escherichia coli Rich Glucose ­salts 17 Bacillus megaterium Rich Sucrose ­salts 25 Streptococcus lac9s Milk 26 Streptococcus lac9s Lactose broth 48 Staphylococcus aureus Heart infusion broth 27 ­30 Lactobacillus acidophilus Milk 66 ­87 Rhizobium japonicum Mannitol ­salts ­yeast extract 344 ­461 Mycobacterium tuberculosis SyntheFc 792 ­932 Treponema pallidum Rabbit testes 1980 Growth Rate g = 0.693 (t  ­ t0)/(ln Nt  ­ ln N0) g = generaFon Fme (h) t0 = Fme zero t = elapsed Fme (h) when sample taken N0 = value at Fme zero Nt = value in sample Value = number, cell component, cell acFvity, turbidity Growth Rate g = 0.693 (t  ­ t0)/(ln Nt  ­ ln N0) g = generaFon Fme (h) t0 = 0 t = 12 h N0 = 107 cells/ml Nt = 109 cells/ml g = 0.693(12 ­0)/(ln 109 – ln 107) = 1.806 h = 108 min Effect of NutriFon on Growth Rate Salmonella typhimurium Medium Feature Growth Rate (g in min) Nutrient broth Very rich 22 All aa Complete mix + salts 32 ParFal aa set 8 aa + salts 49 Glucose minimal Glucose, NH3 + salts 50 Succinate minimal Succinate, NH3 + salts 64 Asp minimal Asp + salts 72 Lys minimal Lys + salts 97 Things that impact growth rate •  Medium c...
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This document was uploaded on 11/11/2013.

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