443 Lecture 15 Growth (1)

Very sensifve if plafng condifons are opfmal

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Unformatted text preview: the number of cells. Method Applica,on Comments Direct microscopic count EnumeraFon of bacteria May not disFnguish living from nonliving cells (tryptan blue) Viable cell count (colony counts) EnumeraFon of bacteria in milk, foods, soil, water, laboratory cultures, etc. Very sensiFve if plaFng condiFons are opFmal Measurement of dry weight or wet weight of cells or volume of cells a\er centrifugaFon probably more sensiFve than Measurement of total cell yield total N or total protein in cultures measurements Measurement of Growth •  1. Direct physical measurement of dry weight, wet weight, volume of cells a\er centrifugaFon, or counts of the number of cells. •  2. Direct chemical measurement of some chemical component of the cells, such as total N, total protein, or total DNA content. •  . Method Applica,on Comments Measurement of total N or protein Measurement of total cell yield from very dense cultures only pracFcal applicaFon is in the research laboratory Measurement of Growth •  1. Direct physical measurement of dry weight, wet weight, volume of cells a\er centrifugaFon, or counts of the number of cells. •  2. Direct chemical measurement of some chemical component of the cells, such as total N, total protein, or total DNA content. •  3. Indirect measurement of chemical ac,vity such as the rate of O2 producFon or consumpFon, CO2 producFon or consumpFon, etc. Method Measurement of Biochemical acFvity e.g. O2 uptake, CO2 producFon, ATP producFon, etc. Applica,on Comments Microbiological as...
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This document was uploaded on 11/11/2013.

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