443 Lecture 15 Growth (1)

Coli eg growing in nutrientrich broth slowgrowing

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Unformatted text preview: hermophilic 25  ­ 45 45  ­ 60 >80 Min Bacteria Opt Max 2 ­5 6.5 – 7.5 8  ­ 11 Acidophiles Alkaliphiles The two worlds of microbiology No oxygen •  anaerobes Oxygen present •  aerobes –  Energy –  Energy •  Sugars to pyruvate •  FermentaFon •  Waste organic •  Sugars to CO2 •  Basic pathways + TCA •  O2 is the e ­ acceptor •  facultaFve anaerobes –  Energy •  Sugars to pyruvate •  FermentaFon •  Waste organic Obligate Pyr to CO2 Basic pathways + TCA O2 is the e ­ acceptor Obligate Log (number of cells) Culture Yield Yield as Cell mass or # Time Growth Yield Cell mass = Ysubstrate * [Substrate] •  Y substrate = Efficiency of converFng a food (substrate) to cell mass –  Linked to type of metabolism –  + Inorganic electron acceptor •  Higher ATP synthesis •  Less substrate needed because more ATP made per substrate –  Bacterium X •  +O2 •   ­O2, + NO3 •  FermentaFve Yglucose = 58 Yglucose =...
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