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Unformatted text preview: e. probability of
contamination is 1:1,000). Prior to sterilization, your medium contains 103 spores/ml of a
specific bacrterium. The specific death rate constant k, for this spore is 0.07 min-1 at 125 0C
while the activation energy for thermal death is 67,700 cal/mole. Prior to sterilization your
medium contains 1 g/l thiamine. The thermal decomposition of thiamine follows first order
kinetics with a rate constant of 0.014 min-1 at 120 0C and an activation energy of 22,000
cal/mole. If you have a batch sterilizer that can instanteneously heat up and cool down the
liquid, calculate the amount of thiamine remaining in the medium after sterilization at 150 ˚C.
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This note was uploaded on 11/13/2013 for the course BME 339 taught by Professor Georgiou during the Fall '11 term at University of Texas.
- Fall '11