E probability of contamination is 11000 prior to

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Unformatted text preview: e. probability of contamination is 1:1,000). Prior to sterilization, your medium contains 103 spores/ml of a specific bacrterium. The specific death rate constant k, for this spore is 0.07 min-1 at 125 0C while the activation energy for thermal death is 67,700 cal/mole. Prior to sterilization your medium contains 1 g/l thiamine. The thermal decomposition of thiamine follows first order kinetics with a rate constant of 0.014 min-1 at 120 0C and an activation energy of 22,000 cal/mole. If you have a batch sterilizer that can instanteneously heat up and cool down the liquid, calculate the amount of thiamine remaining in the medium after sterilization at 150 ˚C. 3....
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This note was uploaded on 11/13/2013 for the course BME 339 taught by Professor Georgiou during the Fall '11 term at University of Texas.

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