Lab 8 Standard Curves and Volumetric Glassware Laboratory -...

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(Yi-Shuan) Rebecca Wang Lab Partner: Tingyi Dong Chemistry 01 Section Z Exp #8 Standard Curves and Volumetric Glassware Laboratory Purpose : The purpose of this experiment was to determine the quantity of Blue #1 food coloring in Blue “Fierce Grape” Gatorade by plotting an absorbance vs. concentration graph. Procedure: Reference: Chem 01 Exp #8 “Standard Curves and Volumetric Glassware Laboratory” Fall 2011 Tufts University. Blue #1 food coloring was used. Data & Observations: Table 1: Absorbance of Different Concentrations of Blue #1 Dye at 630 nm Sample of Blue #1 Dye Concentratio n (mg/L) Absorbance at 630 nm Sample #0 Water 0.000 Sample #1 1 0.134 Sample #2 2 0.224 Sample #3 4 0.501 Sample #4 6 0.783 Sample #5 8 1.033 Table 2: Absorbance of Different Concentrations of Diluted Gatorade at 630 nm Sample of Gatorade Absorbance at 630 nm Concentrated Sample 1.212 1/10 dilution 0.081 1/4 dilution 0.133
Observations: For the blue #1 food coloring, as the concentration increased, so did the absorbance.

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