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BIBC100-FINAL-KEY

BIBC100-FINAL-KEY - i Name(last first EiIBC 1GB University...

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Unformatted text preview: i Name (last. first) : EiIBC 1GB University of California. San Diego. Dr. Lukas Euehler Summer os FINAL EXAM {ED POINTS} YOUR SCORE: POINTS; YOUR lCLASS GRADE _ All Students please read the following waiver: By signing this wail-fer. I give permission for this exam to be left for me to pick up in Bonner Hall 2235. If I do not sign this waiver. I acknowledge that my exam will be available for pioltup during office hours or class. .I' Signature Date PLEASE READ THE QUESTIONS CAREFULLY BEFORE ANSWERING! i. List all residues required for the catalytic triadfdyad of: 4 points Chmotrypsin: M M 5 ELI: i-iI'Ivr protease: Aid? fijP 2. Which types of amino acid residues fit into the chmotrypsin specificity pocket“? 4 points ’Iwui k2 aid-WAZRQ 3. Give a definition of the Michaelis-Menten constant. 5 points Baha'iffiix Emmi‘fl‘ifl eh "SI/wax 4. Hemoglobin tetramers can switch between a T state and an R state. Indicate for each of the following changes if the number of T state tetrarnols will increase or decrease: 6 points - lowering the partial oxygen pressure IV'O’W-fiI 3“ - lowering the pH {W511 - lowering the C02 concentration J1 W3 “E / 2 Name (last, first) : 5. Draw a calcium — calmndulin binding curve; shew dissociah - - nstant (KI) = luM}. Is this system ever in saturaticn under physiulugical ccnditicnsl‘ 6 pcints -M t?“ n= W”! EH] t5. Gramicidin A is an antibictic peptide. 5 pcints [-icw wculd ycu best describe the mechanism cf this peptide? WINK; a... iwm‘fi-fi‘wmfi‘” 'i What is the scccndan.r structure of this peptide? 8 Lin/LI X 'ii What is the quatematy structure cf a functional peptide? OLI W5 i: 3 Name (last, first) : T. 1|What is the purpose of a hfdl‘flpfltl'ty plot analysis for the nicotinic acetylcholine receptor? 3 points l1 lam/H L794.» {L641 eye?“ ”A 2%me 4.0 maul-(21am 43¢ WM-Qé" infill”) Why would this analysis tool fail if applied for the same purpose to sequences of bacterial porins‘? 2 points TWP; lJLnUA E." Wk CW»! [ken-t ”flak/{ist— ill-Mil) we lam? my lAtJflh flrgafil} I}. Mex—a lNJ‘QeOVLUéjCH til-terminal ends, the approximate location of the acetylcholine binding site, and the segment containing all channel lining amino acid residues. 5 points 4 Name (last, first] : 9. What is the approximate time scale for a helix to coil transition or folding of small peptides and what distance {amplitude} can an atom travel tinting this time span? 4 points i -~ It}? a “Mummy? :1 Warm/smash f, I ll]. 1|What are the six basic steps (intennediates) in a protein folding pathway,r for a dimeric protein? 6 points i. naohaiira i ?. Ct’kbmjfiiith 7.Wd’ii-PA l 1. indicate in what type of solvent flte following non-covalent interactions [binding between site A and E} contribute to the stabilityr of a peptide dimer: 6 points Hydrflphflbic contacts: M Pdi *1 i“ Hydrogen bond: M Luih hit/LN In. Inn pair: [ti i 1"" 4 5 Name (last, first} : 12. How is circular dichroism Spfldfl’flfitflpy (CD) useful to measure the progression of the protein folding paths-tallr for bacterial porios? 4 points 13. Discuss the difference in the amounts and distribution of polar. charged, and hydrophobic amino acid residues on the surfaces of heat stable enzymes in comparison with enzymes of E.co|i. . 5 points 6 Name (last, first} : 14. What is the name of the domains found in antibody proteins? 5 points swam Jagger (law) How many domains are found in a gamma heavy chain? 1% Some domains are called variable domains. How many variable domains are tbund in a functional antibody protein? How many variable domains are needed to bind one antigen molecule?I 2 How many CDR loops are involved in binding one antigen molecule? 6 15. 1|What is the energy source of molecular motors driving: 4 points sliding filaments: ALT“? the ATP synthase: @ M) 16. Name three differences between the nicotinic and musearinic acetyleholine receptor. You can use fimctional and structural differences. #5 points MiG/xii Lm MW ...
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