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Problem_Set1, bimm 100

Problem_Set1, bimm 100 - reaction which required template...

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BIMM100 Fall 2008 Problem Set 1 1. You’re a dedicated student working on fruit flies in the lab. In order to determine its base composition, you extract the flies’ DNA and assay for the concentration of each of the bases. Unfortunately, you drop three of the tubes and only find that [A] is 35% of the total DNA. a. What percentage are the other bases (G, C, T) found in the fly genome? b. What are the ratios of purines to pyrimidines? 2. Do the following conditions cause the Tm of a given strand of DNA to increase or decrease? Explain. a. CGTTAAT Mutating the first two base pairs from CG to AT GCAATTA GC TA b. Raising the pH from 7 to 13. c. Cutting the concentration of MgCl 2 in solution by ± . 3. True or False? Explain. a. It is possible for DNA to form secondary structures.
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b. Chargaff’s rules invalidated the tetranucleotide theory. 4. For the Kornberg experiment in which DNA polymerase was discovered, we talked about a
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Unformatted text preview: reaction which required template DNA, dNTPs and DNA polymerase to replicate the DNA. a. Name three other enzymes which are missing from this simple picture and explain their function in DNA replication. b. What are dNTPs? c. DNA replication proceeds in the 5’ to 3’ direction. Draw the mechanism for how a dNTP is added to the 3’ end of the strand. d. For the following replication fork, draw the leading and lagging strands. 5. What is the cause of DNA supercoiling during DNA replication? What enzyme relieves this supercoiling? 6. As a protein involved in DNA repair in a human skin cell, you and your family are responsible for identifying mismatched bases. 5’ GGCTAGT 3’ 3’ CCGGTCA 5’ a. For the above DNA helix, how can you determine which strand of the helix contains the incorrect base? b. Answer this question if instead you worked in prokaryotic cells....
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