Problem_Set4, bimm 100

Problem_Set4, bimm 100 - BIMM100 Fall 2008 Problem Set 4 1....

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
BIMM100 Fall 2008 Problem Set 4 1. You want to determine which genes are turned on due to the addition of a DNA damaging drug. a. What technique will allow you to do so? b. Describe two methods utilized to obtain the DNA which will be placed on the chip. c. How must you modify the cDNA obtained from the mRNA in order to measure gene expression in each condition? 2. Gene Inactivation a. True or False. If you are working with a yeast strain that is diploid, is it possible to use homologous recombination to knock out an essential gene? Explain. b. If instead you decide to use a regulatable galactose promoter to study the function of an essential gene, why must you perform your experiment within a few hours of shifting the cells to glucose rather than two days later?
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
c. Homologous recombination in higher eukaryotes follows a similar procedure to that used in
Background image of page 2
Background image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 01/10/2009 for the course BIMM bimm 100 taught by Professor Dr.sato during the Fall '08 term at UCSD.

Page1 / 3

Problem_Set4, bimm 100 - BIMM100 Fall 2008 Problem Set 4 1....

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online