{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Problem_Set4_Answers, bimm 100

Problem_Set4_Answers, bimm 100 - BIMM100 Fall 2008 Problem...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
BIMM100 Fall 2008 Problem Set 4 1. You want to determine which genes are turned on due to the addition of a DNA damaging drug. a. What technique will allow you to do so? microarray b. Describe two methods utilized to obtain the DNA which will be placed on the chip. You can either PCR 100-1000bp regions of each gene, or you can design small oligos that are complementary to each gene. c. How must you modify the cDNA obtained from the mRNA in order to measure gene expression in each condition? You need to add a fluorescent dye to each pool of cDNA, each a different color. 2. Gene Inactivation a. True or False. If you are working with a yeast strain that is diploid, is it possible to use homologous recombination to knock out an essential gene? Explain. True. You can knock out one copy of the gene while leaving the other intact, which will be sufficient to allow cell survival. b. If instead you decide to use a regulatable galactose promoter to study the function of an
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern