Lab_Manual_Experiment_2.docx - 1 In-Silico Method Fir...

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In-Silico Method Fir Analyzing Restriction Digests Introduction The present practical reflected an in-silico (computational) approach to study the effect of different restriction endonucleases in terms of restriction digests in linear lambda DNA. Table 1: Restriction Digests with Different Restriction Enzymes: Fragment Sizes Table 1a: Lambda - digested with:EcoR1 # Ends Coordinates Length (bp) 1 (LeftEnd)-EcoRI 1-21226 21226 2 EcoRI-EcoRI 31748-39168 7421 3 EcoRI-EcoRI 39169-44972 5804 4 EcoRI-EcoRI 26105-31747 5643 5 EcoRI-EcoRI 21227-26104 4878 6 EcoRI-(RightEnd) 44973-48502 3530 # Ends Coordinates Length (bp) 1 (LeftEnd)-EcoRI 1-21226 21226 2 EcoRI-EcoRI 21227-26104 4878 3 EcoRI-EcoRI 26105-31747 5643 4 EcoRI-EcoRI 31748-39168 7421 5 EcoRI-EcoRI 39169-44972 5804 6 EcoRI-(RightEnd) 44973-48502 3530 Table 1b: Lambda - digested with: BamHI # Ends Coordinates Length (bp) 1 BamHI-BamHI 5506-22346 16841 2 BamHI-BamHI 34500-41732 7233 3 BamHI-(RightEnd) 41733-48502 6770 4 BamHI-BamHI 27973-34499 6527 5 BamHI-BamHI 22347-27972 5626 6 (LeftEnd)-BamHI 1-5505 5505 # Ends Coordinates Length (bp) 1 (LeftEnd)-BamHI 1-5505 5505 2 BamHI-BamHI 5506-22346 16841 3 BamHI-BamHI 22347-27972 5626 4 BamHI-BamHI 27973-34499 6527 5 BamHI-BamHI 34500-41732 7233

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