bio lab 1 - Biology 05LA Winter Quarter 2008 Lab 1 page 1...

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Biology 05LA – Winter Quarter 2008 Lab 1 – page 1 LAB 1 : INTRODUCTION TO LIGHT MICROSCOPY I: THE COMPOUND MICROSCOPE: ITS CARE, COMPONENTS AND PRINCIPLES OF USE. I. MICROSCOPE CARE The microscope is a precision instrument. It commands the care and respect shown all precision equipment. Microscopic studies have their optimum value only if the instrument is maintained in prime condition and is used correctly Microscope care is a major concern of this week’s exercise. The microscopes we will use in this course (and all such instruments) are easily damaged and do not work well, if at all, when damaged. We will provide you with a few simple guidelines throughout this exercise to help you to avoid damaging your microscope and compromising the results of your microscopic studies. II. MICROSCOPE COMPONENTS A discussion of the parts of the microscope will facilitate its use and maximize its value as a scientific tool. Figure 1 shows the parts of our microscopes - the Nikon Alphaphot. At this point, you should obtain a microscope for use in today’s lab exercise. Please see item # I . in the SUMMARY OF MICROSCOPE USE PROCEDURES on page 6 for instructions. Once you have a microscope, take a few minutes to look at the diagram to the right and familiarize yourself with the names of the parts - you should to return to this diagram as often as necessary so that you can learn these names. Structural elements . 1. Binocular eyepiece tube . This complex assembly holds the ocular lenses. It is designed to allow adjustment of the distance between the oculars and the focus of one of them. A prism is located internally. The eyepiece tube is held in position by a setscrew (not shown). Loosening of this screw could result in the eyepiece tube falling from the microscope and being damaged. This screw should remain tightened at all times and not used for changing the position of the eyepiece tube. 2. Revolving Nose Piece . This component is attached to the upper part of the microscope arm just beneath the binocular. The objective lenses are threaded into this structure. The nosepiece rotates in an indexed manner that allows objectives of different power to be “clicked” into position in the optical path. Notice that there are grooves on the edge of the nose piece. These were included in the design to allow it to be rotated easily. Course focus Brightness control Illuminator Arm Diopter ring Base Fine focus Objective lens (1 of 4) Stage motion controls Condenser Mechanical stage assembly Mechanical stage assembly Condenser focus Binocular eyepiece tube Condenser diaphragm lever Oculars (10x) Revolving nosepiece Figure 1. Parts of the compound light microscope; The Nikon Alphaphot.
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Biology 05LA – Winter Quarter 2008 Lab 1 – page 2 3. Arm . The vertical support that constitutes the major structural element of the microscope. The arm is grasped firmly when the microscope is moved. 4.
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This note was uploaded on 04/07/2008 for the course BIO 5A taught by Professor Zhu/cardullo/rao during the Winter '08 term at UC Riverside.

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bio lab 1 - Biology 05LA Winter Quarter 2008 Lab 1 page 1...

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