Microbiology Day 20 - Microbiology Day 20 There is a...

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Microbiology Day 20 There is a repressor protein similar to lac system. Mobile Genetic Elements Slide 2 IR= inverted repeats and all mobile genetic elements have a transposase gene. But the transposons have extra stuff and has its extra stuff that brings along vancomyocin resistance. There’s different things and ligase is what adds to macromolecules. So this allows difference in peptoglycan monomer. Slide 3 In bacteria with have insertion sequences (IS) elements trasnposons and other derivatives where they do similar function. They all have transposase genes that allow for certain functions. So there’s an insertion elements (transposase) and its essential a chunk of DNA that can move from place to place in its own genome and to other genomes. Slide 4 A transposon has transposase and other genes as well. In this case, tn5 has kanomyocin resistant gene and when it gets transferred, it takes the ability to confirm resistance to the antibiotic. These are more like blocks where one has resistance vs the other. It doesn’t have to have antibiotic resistant and can be pathogenicity islands, so it doesn’t have to be antibiotic resistance. So here, it has two insertion elements, and the gene is on the right side and left side and has been mutated. What would happen if insertion elements move from place to place? It will insert in the middle of the gene and disrupt it in the recipient and create a mutant. They will bring with it other function and rarely, it can also act as a promoter. SO it can insert itself in front of an inactive gene and turn that gene on. So insertion elements can only cause happen or pull out some DNA from host, so it can move host gene from one to the other. So transposons bring with it other phenotypic characteristics. Slide 5 Difference between composite and non-composite transposon? They both have a transposes, but the transposases are different. Remmeber Tn5 has transposas at the other side. If we look at IR sequence, we can see, at the end of composite, are two separate IS elements and non- composite have inverted repeats, so it’s like IS elements with added insite there? On composite we have IS elements IS, but with non-composite, they’re just inverted repeats. There’s other things, but that all we will talk about. Bacteriophage Mu has all the genes associated with it and head deteremines the host range, it has IR and we can see lytic and lysogenic gene. Slide 6 Mechanism:
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Conservative transposition, its excised from one location to the other, so the number of genetic elements is still the same.
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