Microbiology Day 12 - Microbiology Day 12 Autoclave common...

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Microbiology Day 12 Autoclave, common way to sterilize, and pressure is used to kill spores, spores when there’s lack of nutrients or bad environment, they make hard shell, where they don’t do anything but stay alive. Slide 3 Sometimes filters are used to filter out and sterilize things and not all things can be autoclaved (not all chemicals). Sometimes sugars are added after and if you have protenation material, you can’t autoclave that. When you add sugar to caramelize, it hardens, so same thing can happen. In that case, you filter stuff. Pore size of 0.2nm is used to filter out most bacteria (99%). What can’t be filtered out is viruses and they were discovered that pathogenic particles couldn’t be seen. Bigger filters 5 nm can be used. There’s also ones that are 0.45 nm and that difference from 0.2 nm is a big deal and some bacteria can flow through. Its common mistakes since they look the same and they don’t label it. 0.2 is the one you can use to sterilize a liquid. If you want to sterilize something viscous, you can’t do it with filter since it gets clogged up. Some water treatment plates, they use reverse osmosis filters. Slide 4 These are kinds of sterilization possible: 1) Bacteriostatic means no change. It tells us that it doesn’t kill bacteria but just stops their growth. Why is that important? A) Less septic shock, which would increase immune reaction way to much. B) Want to protect natural flora, so if you use high level of antibiotic, you might kill them as well. C) The immune system has enough time to reactive and create its own response and it can take care of the bacteria itself. D) Also protects the host and likely has less effect on the host. If something killed the bacteria, it’s probably a stronger system. Most of the concentrations used fall in this case. 2) Bacteriocidal kills the cells. So there’s a bunch of dead cells present (so doesn’t lyse them) 3) Bacteriolytic lyses the cells so total cell count goes down as well, like penicillin. You get this minimum inhibitory concentration (MIC). That means that it’s the concentration that inhibits growth (not killing it). In these tubes, we have a gradient of concentration for antibiotic.
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