Microbiology Day 13 - Microbiology Day 13 You test for MIC...

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Microbiology Day 13 You test for MIC in the lab, but that isn’t the same as in the host. There’s a lot of molecular interactions , where the target is and what it is , and delivery . You may have to increase concentration to get it across our membranes. What if we have a culture, we put in test tube, put antibiotic, shake it and let it sit, and you get good cell death. In the host, there’s hardly any cell death. You try everything, so if the bacteria in blood and you put in the MIC, but a lot of cells aren’t actually dying? 1) If they are clumped together, you might kill one batch, but underneath is protected. If they form a film, the antibiotic can’t get behind there (part of delivery). So we have normal clumping or film (the bacterial that form under and are harder to kill). 2) Processing, so antibiotic processing. The host enzyme has to act on antibiotic to make it active. Sephirol works better in bladder in presence of urine since it gets activated. A lot of bloods we take and the form you take it is not active and has to be converted into something. Many antibiotics have to be chemically changed into active forms. This is why lab generated MIC is not the in situ MIC (along with the three reasons form last lecture) Slide 11 These are some of ways to get resistance, phosphorylation adenylation and it can also affect drug. B-lactamase and some of changes you do to drug don’t always have to do with killing target. Some other R group changes affects the way binds. So ampicillin comes in to transpeptidase, cell doesn’t work and cell dies. Or, we have B-lactamase so the target isn’t affected. However, I can change the way that the analog binds, so it can’t bind to B-lactamase and binds to another drug. Various ways to get resistance: 1) Lack of target 2) Impermeable to the substance, so it has to transport and has to get across. Sometimes, we have these proteins that pump out toxins and the more toxins, the more NDR we have 3)
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