CM1402‐TUTORIAL 6 (Week 10)Question 1:The distribution constant for X between n‐hexane and water is 9.6. Calculate the concentrationof X remaining in the aqueous phase after 50.0 mL of 0.150 M treated by extraction with thefollowing quantities of n‐hexane:a)One 40 mL portionb)Two 20 mL portionsc)Four 10 mL portionsd)Eight 5 mL portionsQuestion 2:Describe the chromatographic technique which can be used to separate the following complexreaction mixtures and explain why:a)Synthesis of 30‐base RNA oligonucleotide sequencesb)Buffer exchange of recombinant protein moleculesc)Synthesis of 10‐mer peptide moleculesd)Isolation of specific enzymes from cell lysate using antibodiese)Preparation of hydrophobic organic‐based pharmaceuticals
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ml portions, reverse‐phase partition chromatography