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Unformatted text preview: chromatography and partition chromatography used in the separation of organic small molecules. What are the advantages and disadvantages of partition chromatography (silica‐C18) compared to adsorption chromatography (silica)? Question 4: A polar organic compound X can be separated using reverse‐phase partition chromatography (HPLC) with a mobile phase of water:acetonenitrile (1:1 v/v) and a stationary phase of silica‐C18 with a retention time of Y min. Rationalize the expected changes to its retention time when the solvent composition is changed to: a) water:acetonenitrile (5:1 v/v) b) water:acetonenitrile (1:5 v/v)...
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This document was uploaded on 12/09/2013.
- Fall '13