# Lab 6.docx - Lab 6 Calibration Methods and Introduction to...

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Lab 6: Calibration Methods and Introduction to UV-Visible Spectroscopy Report Sheet CHEM 2800L Prelab Questions 1. a. (5) You will create 1.000L of 100.0 ppm stock solution of Acetylsalicylic acid (ASA, from the pure powder) in a 2(v/v)%HCl in this lab. 2(v/v)% HCl means the volume of concentrated HCl and the volume of the diluted HCl is 2:100. Determine the mass of the solute and volume of conc. HCl you are going to weigh out or measure with a graduated cylinder. Show your calculation. V = 1 L C = 100 ppm --> 100 mg/L mass = 100 mg for final concentration of HCL --> 2% add 100 mg of Acetylsalicylic Use a 2 v/v stock solution of HCl so, measure: 1 Liter of mix + 100 mg of ASA so this will be 2% in HCl and 100 ppm for ASA b. (5) Describe exactly how you will make the 1.000L of 100.0 ppm ASA. When this is weighed out, you must get the mass you have found exactly! 100 mg/L x 1 L = 100 mg 0.100 g is needed 2. (5) Create dilution tables to create your standards for the external calibration method given a 100.0 ppm solution and a 10.00 mL volumetric flask and a micropipette with a range from 100.0 to 1000 μL. Use C1V1= C2V2. Solution s Sol Conc (ppm) Vol used (mL) Diluted Vol (mL) Final Sol Conc (ppm) A 1000 (given) 5.00 10.00 50.00 B sol A 5.00 10.00 25.00 C sol B 5.00 10.00 12.50 D sol B 5.00 20.00 3.125 E sol C 5.00 10.00 6.25 3. (5) Create a procedure and dilution for a 325 mg/tablet ASA to be placed in a 1.000 L volumetric flask and then diluted to 10.00 ppm in a 10.00 mL volumetric using a micropipette.
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