Protocol 4.2

Protocol 4.2 - Protocol for Group 9 Kurt Ed Shane 1....

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Protocol for Group 9 Kurt Ed Shane 1. Casting of agarose gene. Melt agarose solution in a microwave and store at 50°C insert a gel comb near one end. Let sit for 10-20 min 2. Sample preparation (just before you are ready to load the gel) Remove the paper covering from a piece of parafilm and with a marker pen label the protected side of a piece of Parafilm™ (the side that had the paper protection). Spot 2 μl drops of Gel loading dye mix onto the parafilm for each PCR reaction. With a fresh tip, add 10 μl of a specific PCR product (US or DS fragment) to a dye spot and mix using the pipettor (draw up several times). 3. Electrophoresis Gently remove comb Carefully remove tape from ends of gel (do not tilt the gel tray during this step). Submerge the gel in 1 x TAE in the gel box such that the buffer overflows over the top of the gel. Using the map, load each of your samples into the wells. Place the gel box lid in the correct position and connect to the power supply
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This note was uploaded on 04/08/2008 for the course BSCI 412 taught by Professor Lee during the Spring '08 term at Maryland.

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Protocol 4.2 - Protocol for Group 9 Kurt Ed Shane 1....

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