Protocol 1.2

Protocol 1.2 - Kurt Lowery Shane Hodges Ed Stanley BSCI412...

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Unformatted text preview: Kurt Lowery Shane Hodges Ed Stanley BSCI412 0102 Group 9 Protocol 1.2 1.2.1 Calculate the percent killing for each UV exposure 1) Determine the amount of cells for each of the T-0 dilutions by counting colonies 2) Determine the amount of cells for each time exposure for each dilution by counting surviving colonies 3) Determine % survival by dividing the surviving amount by T-0 Sorry, for the charts and graphs you have to make, you need to find the % survival not % killed. You will have to calculate the % survival for the other samples by calculating the concentration of each sample (CFU/ml) and than divide each one of these by the concentration of time 0 and multiplying by 100. 1.2.2 Calculate the MOI in the selection of the spontaneous lambda resistant mutations 1) MOI = # of phages/ # of cells 2) Determine the amount of phages were placed in each well 3) Determine the amount of cells in each dilution 4) Divide amount of phages inoculated by total amount of cells 1.2.3 Calculating the Frequency of Spontaneous Mutations to -Resistance1....
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This note was uploaded on 04/08/2008 for the course BSCI 412 taught by Professor Lee during the Spring '08 term at Maryland.

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Protocol 1.2 - Kurt Lowery Shane Hodges Ed Stanley BSCI412...

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