Protocol 1.3

Protocol 1.3 - Kurt Lowery Shane Hodges Ed Stanley BSCI412...

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Unformatted text preview: Kurt Lowery Shane Hodges Ed Stanley BSCI412 0102 Group 9 Protocol 1.3 1.3.1 Calculating the Frequency of UV induced mutations to lambda resistance for each exposure time 1) Calculate PFU 2) Calculate CFU 3) Calculate MOI by dividing PFU by CFU 4) Determine number of resistant colonies 5) Divide the number of mutants by the number of cells 1.3.2 Purifying the Tentative UV-induced lambda resistant mutants This procedure is essentially identical to protocol 1.2.4. 1) Take five or six agar plates, and divide them into fourths. 2) Streak purify each quadrant by using 3 toothpicks per quadrant to streak. Part of the previous streak should be used in the next one. Each quadrant should have a streak that looks like a Z when it is finished. Keep each quadrants streaks separate. 3) Incubate plates at 37 degrees Celsius until next laboratory. 1.3.3 Classification of Streak-Purified Spontaneous -Resistant Mutants 1.3.3.1 Determination of Maltose Utilization 1) To determine whether the -Resistant mutants grow on maltose, the group...
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Protocol 1.3 - Kurt Lowery Shane Hodges Ed Stanley BSCI412...

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