protocol lab 10 biosci 0050

protocol lab 10 biosci 0050 - Protocol Lab #10 11/13/07...

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Protocol Lab #10 11/13/07 Chromatography Purpose: To identify the various pigments used in photosynthesis and their function. Hypothesis: If pigments from a photosynthetic plant are tested by paper chromatography, then those pigments which are smallest and most non-polar will move the most on the paper. Null Hypothesis: If pigments from a photosynthetic plant are tested by paper chromatography, then size and polarity will have no effect on the pigments’ movement on the paper. Predictions: 1) If there is Carotene present, it will move the farthest along the paper. 2 & 3) If Xanthophylls are present, two bands of pigment will form, one higher than the other, but both lower than the Carotene. 4) If Chlorophyll a is present, it will move less than both the Carotene and the Xanthophylls. 5) If Chlorophyll b is present, it will move the least of all the pigments. Rationales: 1) Carotene has the smallest size and least polarity of all the pigments and so therefore will move the most. 2 & 3) Xanthophylls are larger and more polar than Carotene and so will not move as far on the
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This note was uploaded on 04/08/2008 for the course BIOSCI 0050 taught by Professor Carollafarve during the Fall '07 term at Pittsburgh.

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protocol lab 10 biosci 0050 - Protocol Lab #10 11/13/07...

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