Unformatted text preview: Experiment 3: Introduction to Chromatography
Report Sheet Kendal Miletich Tues, 8:30am
9/15/2020 Lab Section_____________
(e.g., Tues, 8am) Part A. Paper Chromatography
Determining Rf values of Food Dyes:
Yellow Spot Distance (cm)
6.3cm Solvent Distance
6.3cm 4.5cm Rf Value
1.0 6.3cm .71 6.3cm 6.3cm 1.0 4.6cm 6.3cm .73 Part B. Determination of an Unknown Food Dye i Letter or Number of Unknown Food Dye Solution: ________________________ Red #3 Identity of Unknown Food Dye (Red #3 or Red #40): ____________________
In the space below, explain in detail how you determined the identity of your unknown food dye.
Provide all relevant data to support your conclusions.
1.) Do the same test as blue, green, and yellow, but with Red #40, Red#3, and the unknown dye.
2.) Take Rf values of each spot.
3.) Compare the values, and whichever red dye is closest to the unknown is the same. 35 Part C. Thin-layer Chromatography
Component Y* ( )
Component Z* ( )
(Tylenol) Spot Distance (cm) Solvent Distance
(cm) Rf Value 3.9cm 4.5cm .87 3.9cm
caffeine 4.5cm .87 3.5cm
aspirin 4.5cm .78 2.2cm
acetominophen 4.5cm .49 3.5cm 4.5cm .78 *The assignment of x, y, and z to a specific compound is arbitrary but correctly correlate your data with the assigned
compound and put the name of the compound in parentheses. Sketch a diagram of your TLC plate from Part C below: (You may skip this TLC diagram step)
Part D. Determination of an Unknown Analgesic 8
Number or Letter of Unknown Analgesic: ________________________
Identity of Unknown analgesic: ____________________
In the space below, explain in detail how you determined the identity of your unknown
analgesic. Provide all relevant data to support your conclusions.
1.) Use same test as in Part C.
2.) Use unknown, exedrin, and salicylamide.
3.) Compare the Rf values to get the unknown. 36 ...
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