Exp3LabRpt- GenChem.pdf - Experiment 3 Introduction to Chromatography Report Sheet Kendal Miletich Tues 8:30am Lab Section Name Date(e.g Tues 8am Part A

Exp3LabRpt- GenChem.pdf - Experiment 3 Introduction to...

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Unformatted text preview: Experiment 3: Introduction to Chromatography Report Sheet Kendal Miletich Tues, 8:30am 9/15/2020 Lab Section_____________ Name____________________________ Date______________ (e.g., Tues, 8am) Part A. Paper Chromatography Determining Rf values of Food Dyes: Dye Blue Green (yellow component) Green (blue component) Yellow Spot Distance (cm) 6.3cm Solvent Distance (cm) 6.3cm 4.5cm Rf Value 1.0 6.3cm .71 6.3cm 6.3cm 1.0 4.6cm 6.3cm .73 Part B. Determination of an Unknown Food Dye i Letter or Number of Unknown Food Dye Solution: ________________________ Red #3 Identity of Unknown Food Dye (Red #3 or Red #40): ____________________ In the space below, explain in detail how you determined the identity of your unknown food dye. Provide all relevant data to support your conclusions. 1.) Do the same test as blue, green, and yellow, but with Red #40, Red#3, and the unknown dye. 2.) Take Rf values of each spot. 3.) Compare the values, and whichever red dye is closest to the unknown is the same. 35 Part C. Thin-layer Chromatography Analgesic (compound name) (Aspirin) Excedrin Component X* ( ) Excedrin Component Y* ( ) Excedrin Component Z* ( ) (Tylenol) Spot Distance (cm) Solvent Distance (cm) Rf Value 3.9cm 4.5cm .87 3.9cm caffeine 4.5cm .87 3.5cm aspirin 4.5cm .78 2.2cm acetominophen 4.5cm .49 3.5cm 4.5cm .78 *The assignment of x, y, and z to a specific compound is arbitrary but correctly correlate your data with the assigned compound and put the name of the compound in parentheses. Sketch a diagram of your TLC plate from Part C below: (You may skip this TLC diagram step) Part D. Determination of an Unknown Analgesic 8 Number or Letter of Unknown Analgesic: ________________________ Salicylamide Identity of Unknown analgesic: ____________________ In the space below, explain in detail how you determined the identity of your unknown analgesic. Provide all relevant data to support your conclusions. 1.) Use same test as in Part C. 2.) Use unknown, exedrin, and salicylamide. 3.) Compare the Rf values to get the unknown. 36 ...
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