zLAB 2 - Lab 2 First Microscopy Lab I Purpose The purpose...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Lab 2: First Microscopy Lab I. Purpose The purpose of this lab is to familiarize the students with the inner workings and proper  use of light microscopes, as well as to introduce certain key terms such as magnification,  resolution, contrast, phase contrast, and brightfield. II. Procedure After viewing a short film on microscopy and identifying the pieces and functions of the  light microscope, students began to use their microscopes using a prepared slide.  They were  instructed to practice using objectives, focusing, and basic microscopy maneuvers, and then set  the Kohler illumination.  Once these steps had been completed, the next phase instructed the  students to calculate the resolution of each objective lens and compare it to the labeled  resolution.  Steps were then undertaken to calibrate the eyepiece micrometer in the microscope  by utilizing a micrometer on a given slide. The conversions were: 4X: 1 mm = 40 retical units 10X: 1 mm = 100 retical units 40X: 1 mm = 400 retical units The next part of this lab involved the examining of an onion root tip.  3 drops of 1N HCl  was added and allowed to soak into a 2 mm piece of onion root tip, and then 3 drops of aceto- orcein solution was added to the tip and 2-3 more minutes were allotted for the root to sit.  A wet  mount of the root tip was then made, coverslip added, and the students began looking through  the microscope hoping to find various stages of mitosis in action. Once at least a few good  examples of the stages of mitosis were found, the slide was taken to a set-up involving an 
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern