Lab Protocol Peroxidase Activity Day 2

Lab Protocol Peroxidase Activity Day 2 - spectrophotometer...

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Materials: Spectrophotometer, pipettes (1 ml, 5 ml, 10 ml), micropepetter, pipette pumps, 10 ml test tubes, Parafilm, test tube rack(s), Kimwipes, transfer pipettes, spectrophotometer tubes, tween20, water Protocol: 1. Turn on spectrophotometer and allow to warm-up at least 15 minutes. 2. Dilute detergent (Tween20) with water to desired amounts. 3. Use pipette to fill test tubes with various amounts of the different solutions, leaving out turnip enzyme. 4. Set wavelength to 500 nm. 5. Zero the meter to “infinite” absorbance. 6. Place “blank” solution into spectrophotometer and set to 100. 7. Put required amount of turnip enzyme into “blank’s” matching solution and place in
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Unformatted text preview: spectrophotometer. 8. Record absorbance/transmittance (how much light is coming through) shown on the spectrophotometer every 20 seconds for two minutes (beginning at 0s). 9. Repeat steps 5-8 with rest of solutions (2x). Data: Tube Guaiacol (ml) 0.1% H 2 O 2 (ml) Phosphate Buffer (ml) Turnip Enzyme Prep (ml) Tween20 (ml) Total Volume (ml) Blank #1 0.1 4.5 1.0 1.0 6.6 Test #1 0.1 0.1 4.4 1.0 1.0 6.6 Blank #2 0.1 4.5 1.0 1.0 6.6 Test #2 0.1 0.1 4.4 1.0 1.0 6.6 Blank #3 0.1 4.5 1.0 1.0 6.6 Test #3 0.1 0.1 4.4 1.0 1.0 6.6...
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This note was uploaded on 04/09/2008 for the course BIOL 113 taught by Professor Moore during the Fall '07 term at Duquesne.

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Lab Protocol Peroxidase Activity Day 2 - spectrophotometer...

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