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LAB EXERCISE 2 – MICROBES, “GOOD” OR “BAD”? Answers OBJECTIVES: Learn to make scientific observations, draw , and describe what you see. Learn the skill of microscopy correlating it with the size of an organism. Learn how microscopes and stains can be used to distinguish among different types of microbes Explain why the identification of pathogenic microbes is important for treating diseases Distinguish between different types of prokaryotic and eukaryotic cells. Identify microbes ( Bacteria, Protists (Protozoan), Fungi ) Use these skills to identify the microbe that caused the “soft rot” in the potatoes (Lab 1) PROCEDURES FOR DOING THIS LAB: Read this lab exercise before coming to class. In class, follow the directions for completing Activities 1-17. In order to finish doing the Activities, try to work within the time indicated at each Activity. Class time will be used to do microscopy work. You will not have time to read the background information. After class, reread all the information in the lab exercise and answer all the questions as homework. Initially, try to answer the questions on your own. Answers will be posted after the last class on Friday, September 14 th . The post-lab quiz for Lab Exercises #1 and #2 will be given at the beginning of class the week of Lab Exercise #3. Slides will be available for review in office hours, G9A Clapp. QUESTIONS ASKED IN THIS LAB: I. What is the difference between genetic diseases, diseases of aging, and infectious diseases? II. Why do we need microscopes to study microbes? III. What are the important parts of a compound light microscope? IV. How do you focus a slide using a compound light microscope? V. How can oil immersion lens increase increase resolution at high magnifications? VI. What do we expect bacteria to look like under the microscope? VII. Can we use a microscope to determine if a bacterium is pathogenic? VIII. How are stains, bacterial cell walls, and microscopes used together to diagnose diseases? IX. Can an electron microscope help us to learn more about bacteria? X. Do bacteria actually contribute to our food products? Or do they only contaminate our food? XI. What are biofilms? XII. Are bacteria really everywhere and if they are, can they cause disease? XIII. Are bacteria in the soil and are they “good” or “bad”? XIV. Have you ever smelled soil? XV. Are there other bacteria that are not round, rods, or spirals? XVI. Are fungi “good” or “bad”? XVII. What is one of the most famous molds? XVIII. Are yeasts fungi? XIX. How can “good” yeast become pathogenic? XX. Are there microbial eukaryotes? XXI. Are some Protists pathogenic? XXII.
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This note was uploaded on 04/07/2008 for the course BIOSCI 0050 taught by Professor Carollafarve during the Fall '07 term at Pittsburgh.

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