FA13+CHEM+225+Exp8+Column+Chromatography_001

4 the component that interacts or binds more strongly

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Unformatted text preview: as the compounds travel through the column. 4) The component that interacts or binds more strongly to the stationary phase moves more slowly in the direction of the flow of the mobile phase. 5) Forces that are involved in binding of the components to the stationary phase: hydrogen bonding, dipole-dipole forces, electrostatic forces, complexation, Van der Waals forces Normal Phase Chromatography. The packing material for the stationary phase is a polar adsorbent, like alumina (Al2O3) or silica gel (SiO2.xH2O). Organic solvents are used for the mobile phase. For this type of system (normalphase) compounds with more polar groups are held more tightly to the stationary phase. Normal-phase chromatography will be used for this experiment. RCOOH, ROH, RNH2, R1R2C=O, R1CO2R2, alkenes, RX !increasing absorption on polar stationary phase Experimental Details Materials. A dry glass column about 1 cm in diameter, >25 cm in length, and fitted with a Teflon stopcock (note that the glass column is referred to as the ...
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