Unformatted text preview: as the compounds travel through the
4) The component that interacts or binds more strongly
to the stationary phase moves more slowly in the
direction of the flow of the mobile phase.
5) Forces that are involved in binding of the
components to the stationary phase: hydrogen
bonding, dipole-dipole forces, electrostatic forces,
complexation, Van der Waals forces
Normal Phase Chromatography.
The packing material for the stationary phase is a
polar adsorbent, like alumina (Al2O3) or silica gel
(SiO2.xH2O). Organic solvents are used for the
mobile phase. For this type of system (normalphase) compounds with more polar groups are held
more tightly to the stationary phase. Normal-phase
chromatography will be used for this experiment.
RCOOH, ROH, RNH2, R1R2C=O, R1CO2R2, alkenes, RX !increasing absorption on polar stationary phase Experimental Details
Materials. A dry glass column about 1 cm in diameter,
>25 cm in length, and fitted with a Teflon stopcock
(note that the glass column is referred to as the
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