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Unformatted text preview: n plug with enough clean, sand to form a layer
about 0.5 cm thick. Add approximately 20 mL of
petroleum ether (Figure 1). Place a dry funnel on top of
the column, and slowly add 7 g of alumina to the
column while gently, tapping the column. A rubber
"tapping mallet" may be made for gentle tapping the
column by placing a glass rod in a rubber stopper.
When this process has been completed, rinse the inner
walls of the column with additional petroleum ether to
remove any alumina that may adhere to the sides. The
tapping mallet may also be used to help level each
layer. Cover the alumina with a 0.5-1 cm layer of sand.
Open the stopcock to allow the solvent to begin to drain
into an Erlenmeyer flask until the solvent level exactly
reaches the top of the alumina. At that point, close the
stopcock. NEVER allow the solvent/sample to drain
below the top of the alumina, as air bubbles and
channels may develop in the solid support. The
“column bed” is now ready for sample “loading.”...
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