FA13+CHEM+225+Exp8+Column+Chromatography_001

Be aware that these videos will show you a general

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Unformatted text preview: ions. Be aware that these videos will show you a general technique but the compounds, solvents, and quantities are not the same as those you will use in the laboratory. Use these videos to become familiar set-ups and procedures, but think carefully about how your experiment may differ from the video. • Column Chromatography – technique: (22 minutes) http://www.youtube.com/watch?v=6fzBJ8nuuzk Note: (1) You will not use a “rotovap” or the method of dry-loading sample as described in 12:10-14:35 min (2) You will use a dry-packing method of preparing the alumina column bed (3) You will use beakers, not test tubes to collect fractions • Column Chromatography – theory: (7+1 minutes) http://www.youtube.com/watch?v=ukOFivS7xhA http://www.youtube.com/watch?v=9GiLjH9Oym8 • Thin-Layer Chromatography – re-assigned: (16 min) https://www.youtube.com/watch?v=EUn2skAAjHk& feature=player_embedded Other Prelab Information • Prepare your notebook and review the Material Safety Data Sheets (MSDS), as usual. In your notebook, you must include a diagram of the column c...
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