New FOR QUES 5 - INH

New FOR QUES 5 - INH - PROCEDURE FOR QUESTION 5: EFFECT OF...

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1 PROCEDURE FOR QUESTION 5: EFFECT OF COMPETITIVE INHIBITOR, HYDROXYLAMINE USE GOGGLES AND GLOVES FOR THIS EXPERIMENT General procedural hints for groups studying the effect of pH on turnip peroxidase: 1. You will follow the procedure you used in Exercise 6 , the baseline experiment (See pages 10-11) but you will use the new Mixing Table. You will need to do a new Baseline experiment using no inhibitor as your baseline- Tubes 2 and 3. You will have to do another baseline because the turnip extract will be different from that made in the previous lab. The baseline run will be the positive control for the variable that you are testing. You will compare all other conditions studied to the baseline condition (Tubes 2 and 3). Note that in this case Tube 1 is your “blank” as well as your “negative control”. You will use Tube 1 to calibrate the spectrophotometer for each set of conditions (2 and 3), (4 and 5), (6 and 7), etc. 2. As part of your protocol, you should refer to the
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New FOR QUES 5 - INH - PROCEDURE FOR QUESTION 5: EFFECT OF...

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