PROC FOR QUES 4 - pH

PROC FOR QUES 4 - pH - PROCEDURE FOR QUESTION 4: EFFECT OF...

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1 PROCEDURE FOR QUESTION 4: EFFECT OF pH General procedural hints for groups studying the effect of pH on turnip peroxidase: 1. You will follow the procedure you used in Exercise 6, the baseline experiment (See pages 10-11) but you will use the new Mixing Table. You will need to do a new Baseline experiment using pH 5 as your baseline - Tubes 2 and 3. You will have to do another baseline because the turnip extract will be different from that made in the previous lab. The baseline run will be the positive control that contains the variable for which you are testing. You will compare all other conditions studied to the baseline condition (Tubes 2 and 3). Note that in this case Tube 1 is your “blank” as well as your “negative control”. You will use Tube 1 to calibrate the spectrophotometer for each set of conditions (2 and 3), (4 and 5), (6 and 7), etc. YOU MUST HAVE A NEW TUBE 1, FOR EACH CONDITION. BE SURE THAT YOU USE THE APPROPRIATE pH FOR THE BUFFER EACH TIME YOU SET UP YOUR NEW TUBE 1. 2.
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This note was uploaded on 04/07/2008 for the course BIOSCI 0050 taught by Professor Carollafarve during the Fall '07 term at Pittsburgh.

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PROC FOR QUES 4 - pH - PROCEDURE FOR QUESTION 4: EFFECT OF...

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