2structuredetermina5onrequiresassignments

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Unformatted text preview: n.fying
helices,
then
iden.fy
β‐strands.

 Notes:
helices
are
easy
to
iden2fy
by
the
characteris2c
i,i+3
mid‐range
NOEs.
Helices
also
have
small
 values
of
J3αH‐NH
and
strong
sequen2al
dNN,
dβN.
β‐strands
are
more
difficult
to
pick
out.
All
 secondary
structure
should
be
rela2vely
protected
(s2ll
observable
aGer
1
h
in
D2O,
although
 strands
at
the
edge
of
a
sheet
will
be
less
well
protected.
β‐strands
are
recognized
by
the
strong
 sequen2al
dαN
and
large
J3αH‐NH.

 2.

Structure
determina5on
requires
assignments.
 a.
What
interac5on
provides
through‐bond
coupling
useful
for
the
assignment
process?
 
J‐coupling
 b.
Name
a
pair
of
NMR
experiments
you
would
use
to
assign
the
backbone
of
a
medium
 sized
13C/15N
labeled
protein.
 
HNCA,
HN(CO)CA




OR







HNCO,
HN(CA)CO
 c.
Draw
a
pep5de
seg...
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This note was uploaded on 01/20/2014 for the course BIOLOGY 5337 taught by Professor Ponder during the Fall '11 term at Washington University in St. Louis.

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