Thismakesnmrinsensi2ve

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Unformatted text preview: ment
and
illustrate
the
through
bond
correla5ons
you
expect
to
 observe
with
each
experiment.
Draw
a
sample
strip
of
the
spectrum
with
the
expected
 resonances
for
each
experiment
for
2
adjacent
residues,
i,
i+1.
 i
 Res
i
 i+1
 O
 O
 H
 N
 Cα C
 N
 Cα C
 HN(CA)CO
 Hα R Hα Cβ HNCO
 Hβ Res
i+1
 H
 i
 i‐1
 i
 13C’
 i‐1
 HNCO
 HN(CA)CO
 1H
 ***OR***
 i
 i+1
 HN(CO)CA
 H
 O
 H
 O
 N
 Cα C
 N
 Cα C
 Hα R Hα Cβ HNCA
 Hβ HNCO
 HN(CA)CO
 1H
 Res
i
 i
 Res
i+1
 i‐1
 i
 i‐1
 HNCA
 HN(CO)CA
 1H
 HNCA
 HN(CO)CA
 1H
 3.
What
does
the
spliSng
between
the
nuclear
spin
energy
levels
depend
on?
Is
this
spliSng
 large
or
small?
How
does
this
contribute
to
the
insensi5vity
of
NMR
as
a
technique?
 ∆E=γBo.
Gyromagne2c
ra2o...
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