B is unfolding a global or local process local 70

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Unformatted text preview: depends
on
the
type
of
nucleus,
Bo
is
the
spectrometer
field
strength.

 This
spliVng
is
small.
Therefore,
the
popula2on
difference
between
the
two
states
is
small
 (Boltzmann
distribu2on)
and
the
net
magne2c
moment,
which
is
the
bulk
magne2za2on
we
can
 manipulate,
is
quite
small.
This
makes
NMR
insensi2ve.
 4.
Do
you
expect
to
see
localized
chemical
shiW
changes
or
widespread
chemical
shiW
in
the
 NMR
spectrum
of
hemoglobin
upon
binding
by
oxygen?
Why?
 You
would
expect
more
widespread
chemical
shiG
changes.
Binding
oxygen
will
cause
shiGs
in
 the
local
binding
site.
However,
hemoglobin
is
allosterically
regulated
...
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This note was uploaded on 01/20/2014 for the course BIOLOGY 5337 taught by Professor Ponder during the Fall '11 term at Washington University in St. Louis.

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