47 reversetranscription

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Unformatted text preview: strand. 40 Topoisomerase ­‑DNAGyrase: Relaxingsupercoilsaheadofthefork •  Unwindingthedouble helix •  aheadoftheforkisthe firststep •  Carriedoutby Topoisomerasewhichisa DNAgyrase 41 KeyDNAReplicationEnzymes 1.  2.  RNAprimase –  Distributive–addsafewnucleotides(8 ­‑12) –  SynthesizesanRNAprimer DNApolymeraseIIIHoloenzyme –  Processive ­‑canaddmanynucleotides –  PolymerizationoffreenucleotidesontheDNAtemplate –  Hastwocatalyticcores •  leadingstrandsynthesis •  laggingstrandsynthesis. –  Actsasadimerinordertocoordinatethesynthesisoftheleading andlaggingstrand. 3.  DNApolymeraseI –  –  Proofreadingfunction DegradingRNAprimer 42 DNAReplicationEnzymes AccessoryProteins. –  Slidingclamp •  EncirclestheDNAlikeadonut •  RetainsDNApolIIIonthetemplateuntilreplicationis complete •  ResponsibleformakingDNApolIIIaproccessive enzyme. –  Bridgingenzymes •  Connectsthetwo...
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