Poli3 5 exonucleaseactivityrecognizesmismatchesand

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Unformatted text preview: ­‑parallel. •  Replicationcanonlyproceedin5 to3 direction. •  Onlyonestrandcancarryoutcontinuoussynthesis. –  Leadingstrand •  Theoppositestrand(Laggingstrand)requiresa differentstrategy. •  becauseDNAsynthesiscannotoccurinthe3 to5  direction. 24 DNAismadeoftwoanti ­‑parallel helices 5 3 3 5 25 TheReplicationFork 26 Problem2 •  DNApolymerizationoccursonlyona primedtemplate Solution:Twostrategiesforthetwostrands 30 DNAreplicationineukaryotes multipleoriginsreplicatechromosomesrapidly 31 Problem4 •  Laggingstrandisdiscontinuous–hasgaps •  Solution •  5  ­‑3 exonucleaseactivityofDNApolymerases 5  ­‑3 exonucleaseactivity (degradestheRNAprimersfromokazakifragmentstomakea continuousDNAstrand) Problem5 Maintaininghighaccuracy Athighspeed DNAReplication: HighAccuracy&HighSpeed •  Veryhighfidel...
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This document was uploaded on 01/18/2014.

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