Topoisomerases

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Unformatted text preview: ity(accuracy). –  Only1errorin1010bases. –  AchievedbytheproofreadingfunctionofDNApolymeraseI 1.PolI3  ­‑5 exonucleaseactivityrecognizesmismatchesand correctsthemduringreplication. 2.PolI5  ­‑3 exonucleaseactivitydestroysRNAprimerofOkazaki fragments •  RNAprimaselackproofreadingfunction. •  ThereforeRNAprimersonokazakifragmentsmayhaveahigh degreeoferrors. •  PolIdegradesRNAprimersthrough5  ­‑3 exonucleaseactivity 36 DNAReplication Solution ­‑3  ­‑5 exonucleaseactivityofDNAPolI DNA Polymerase I and III •  DNA polymerase I –  5’ ­ 3’ DNA polymerase activity, on a primed DNA template –  3’ ­ 5’ exonuclease activity – proofreading for mutations –  5’ ­ 3’ exonuclease activity – degrading...
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This document was uploaded on 01/18/2014.

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