Lecture-6-RNA-modifification

Hbii52 is a brainspecigic cd box snorna containing

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Unformatted text preview: ceptors –  Neuronal death Who guides alternative splicing and RNA editing in humans? Small nucleolar RNAs (snoRNAs) The expanding snoRNA world. Bachellerie JP, Cavaillé J, Hüttenhofer A. Biochimie. 2002 Aug;84(8): 775 ­90. •  Non–protein ­coding RNAs encoded in introns of other genes –  60 to 300 nucleotides (nt) in length –  C/D box snoRNAs – catalyzes 2' ­O ­methylation –  H/ACA box snoRNAs – catalyzes pseudouridylation •  Guides modiGication and processing of non ­coding RNAs –  ribosomal RNAs (rRNAs) –  small nuclear RNAs (snRNAs) –  tRNAs •  Can guide alternate splicing of coding RNAs •  Can guide RNA editing on coding RNAs SnoRNAs (assist in RNA processing) •  Methylation – C/D box snoRNAs •  Pseudouridylation – H/ACA box snoRNAs C/D box snoRNAs •  Guides site speciGic methylation of ribose –  targets rRNAs –  Heavily methylated RNA is protected from hydrolysis. –  rRNA acts as a ribozyme by catalyzing its own hydrolysis and splicing •  Two short conserved sequence motifs –  5 C box = (UGAUGA) –  3 D box = (CUGA) •  •  •  Stem ~ 5 nucleotides upstream C box and downstream D box. D box – Upstream conserved region of 10 ­21 nucleotides ...
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This document was uploaded on 01/18/2014.

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