Lecture-8-RNA-Transcription regulation -Eukaryotic

a graduate student needs to genetically engineer a

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Unformatted text preview: at body speciJic cis acting element Fat body speciJic cis acting element Gene 6 Ovary speciJic cis acting element Gene 1 RNA 1 Ovary speciJic cis acting element Fat body speciJic transcriptio n factor Gene 1 Gene 10 Fat body speciJic cis acting element Gene 6 RNA 6 32 SPATIALREGULATION: WheretoturnthegeneON •  dppgene •  ~10 ­‑100enhancers •  dispersedwithin~50,000bpintervalofDNA. •  Locations –  Most ­‑5 upstreamregion –  Introns –  3 UTR. –  3 downstreamregion •  Differentenhancersetsregulatetheexpressionofthisgene atdifferentsitesinthedevelopingembryo. 33 SPATIALREGULATION: WheretoturnthegeneON VM1 & VM2 activates dpp in the embryonic visceral mesoderm and precursor of the gut musculature LE activates dpp in the lateral ectoderm ID1 & ID2 activates dpp in the imaginal discs 34 HomeoticTransformation 1.  Enhancersarefunctional modules Wildtype Mutant 2.  Fusionofregulatoryelements ofonegene 3.  withtheprotein ­‑codingor structuralgeneofanother gene 4.  resultsintheHOMEOTIC transformation 5.  Ofonepartofthebodyto becomeanotherpartofthe body Partofthethoraxis convertedintoaposterior abdominalsegment 35 HomeoticTransformation Wildtype Mutant 1.  Enhancersarefunctionalmodules 2.  Fusionofregulatoryelementsof onegene 3.  withtheprotein ­‑codingor structuralgeneofanothergene 4.  resultsintheHOMEOTIC transformation 5.  Ofonepartofthebodytobecome anotherpartofthebody Wild ­‑type ­‑apairof imaginaldiscsantennae  Antennapediamutant discs ­‑differentiateas legs 36 Practice Exam Question A graduate student needs to genetically engineer a fruit Lly with wings coming out from its ears. Which of the following cis and transacting elements can he move around the fruit Lly genome that will enable him to do that? 37 Practice Exam Question 1 A graduate student needs to genetically engineer a fruit Lly with wings coming out from its’ ears. Which of the following Cis and/or Trans ­acting elements can he move around the fruit Lly genome that will enable him to do that? Ear Ear ­speciJic transcription factor Enhancer 1 Enhancer 2 Enhancer 3 Enhancer 4 Enhancer 5 Ear ­TF Ear Gene Wing Gene Eye Gene Wing Wing ­speciJic transcription factor...
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