Lecture-8-RNA-Transcription regulation -Eukaryotic

Locations most5 upstreamregion introns 3 utr 3

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Unformatted text preview: onbyRNApolII •  EnhancedtranscriptionviaGC ­‑richboxandCCAATbox •  Evolvedacomplexnetworkwhereaminimumnumberofcis andtransactingelementscanregulateallthegenes –  Furtherenhancementbyenhancersandsilencers 26 TranscriptionFactors LargeRepertoirewithsmallnumberofelements •  TranscriptionfactorsareModular –  FewDNAbindingdomains •  bindingspecificcisactingelements –  Associatewithavarietyoftransacting domain •  activation/repressiondomains –  Cofactors •  canenhanceordiminisheffects •  Mixingandmatching –  smallnumberofDNAbindingdomains+activation/ repressiondomains+co ­‑factors –  Cangenerateamillionsofgeneexpressionpacerns 27 β ­Interferon Enhanceosome •  The β ­Interferon gene –  codes for an anti ­viral protein •  Default state –  OFF state •  Viral infection –  ON state –  assembly of enhanceosome 28 β ­‑InterferonEnhanceosome DNA ­‑protein interactionbetweena helix ­‑turn ­‑helix transcriptionfactor 29 SPATIALREGULATION: WheretoturnthegeneON •  Tissue ­‑specificactivationofgenes –  Enhancers/Silencers –  Transcriptionfactors •  Vitellogeningene –  Makesvitellogenin ­‑eggyolkprotein –  adultfemale–ONinovaryandfatbody. –  twodistinctenhancers •  Ovaryspecificenhancer/transcriptionfactor •  Fatbodyspecificenhancer/transcriptionfactor 30 Fat body speciJic cis acting element Ovary Fat body speciJic cis acting element Ovary speciJic transcriptio n factor Fat Body Fat body speciJic transcriptio n factor Ovary speciJic cis acting element Ovary speciJic cis acting element Ovary speciJic cis acting element Fat body speciJic cis acting element Fat body speciJic cis acting element Ovary speciJic cis acting element Vitellogenin gene Vitellogenin gene Ovarian gene 2 Vitellogenin gene fatbody gene 2 31 Ovary needs RNA 1 +10 Ovary speciJic transcriptio n factor Fat Body needs RNA 1 + 6 Fat body speciJic cis acting element Ovary speciJic cis acting element RNA 1 Ovary speciJic cis acting element Gene 10 RNA 10 F...
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This document was uploaded on 01/18/2014.

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