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Carriedoutbyrnapolymerases 1

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Unformatted text preview: tes: Transcription in Eukaryotes: Splicing of introns Transcription Transcription Transcription: An Overview Transcription: An Overview RNA­like strand RNA transcription RNA transcription Requirements 1. 2. 3. 4. 5. Template to copy from. 1. DNA replication also needs template. Only one strand is used as template (template or non­coding strand) 1. DNA replication uses both strands as templates. Does not need to be primed 1. DNA replication can happen only on primed templates. Carried out by RNA polymerases 1. DNA replication is carried out by DNA polymerases. Starts at specific sequences in the genome = promoters 1. DNA polymerase start requirements are much less specific­11­base recognition sequence and AT­rich repeats. RNA transcription RNA transcription Requirements 1. 2. 3. 4. 5. Requires unwinding of helix and helicases. 1. Same as DNA Only a local effect. Bubble formed extended 500 bases­5 kb 1. DNA forms a replication fork that moves along entire length of chromosome. Transcription always occurs in the 5’ to 3’ direction 1. Same as DNA replication. Addition of nucleotides to the 3’­OH 1. Same as DNA. Complementary base pairing. A­U and C­G bonding 1. Same as DNA for rules but A­T in DNA and A­U RNA. Transcription: Transcription: Different genes can be transcribed in different directions Transcription initiation: Transcription initiation: prokaryotes • • • s subunit A part of the...
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